Vasoactive Intestinal Peptide Enhances Dopamine Accumulation in Primary Cell Culture of Rat Hypothalamus

KOZO KADOWAKI; KOJI KOIKE; KENJI HIROTA; MASAHIDE OHMICHI; HIROHISA KURACHI; AKIRA MIYOKE; OSAMU TANIZAWA

doi:10.1507/endocrj1954.39.197

KOZO KADOWAKI, KOJI KOIKE, KENJI HIROTA, MASAHIDE OHMICHI, HIROHISA KURACHI, AKIRA MIYOKE, OSAMU TANIZAWA

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Keywords: Vasoactive intestinal peptide, Dopamine uptake, cAMP, Hypothalamus

JOURNAL FREE ACCESS

1992 Volume 39 Issue 2 Pages 197-202

DOI https://doi.org/10.1507/endocrj1954.39.197

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Abstract

The effect of vasoactive intestinal peptide (VIP) and PHI-27 on dopamine accumulation in cultured rat hypothalamic cells was investigated. VIP enhanced [³H] dopamine accumulation dose dependently. This effect was siffnificant at 10^-8-10^-5M VIP with a concomitant increase in intracellular cyclic AMP (cAMP), and reached its plateau level at 10^-6M VIP. VIP increased [³H] dopamine accumulation significantly within 15 min. PHI-27 and dibutyryl cAMP ((Bu)₂-cAMP) also enhanced [³H] dopamine accumulation. These results suggest that VIP enhances dopamine accumulation in hypothalamic cells by increasing intracellular cAMP.

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