Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
Original
Variation in Serum Creatine Phosphokinase Activity as Indicated in Two-Phase EMC-D Virus-Induced Myocarditis
Masato MIZUTANIKensuke HIRASAWAMakio TAKEDAKunio DOIMasayoshi YUKAWAYasunobu MATSUMOTOYoshitsugu MATSUMOTOTakashi ONODERA
著者情報
キーワード: CPK, EMC virus, histopathology, myocarditis
ジャーナル フリー

1996 年 45 巻 4 号 p. 333-338

詳細
抄録

In this study, myocardial damage in the D-variant of encephalomyocarditis (EMC-D) virus-induced myocarditis has been investigated consecutively by measuring serum creatine phosphokinase (CPK) activity. CPK activity in 8 week-old male BALB/cAJcl mice inoculated with EMC-D virus increased to a peak at 4 or 5 days postinoculation (DPI) and then gradually decreased. The CPK activity rose again after 7 DPI until it reached a second peak. In view of the kinetics of CPK activity, two-phase (early and late phase) myocardial damage in EMC virus infection were considered. In the late phase, an increase in cellular infiltration in the myocardium and a decrease in viral titer in the heart were observed. It was therefore suspected that the increase in CPK in the late phase may be caused by cellular infiltration, but not by viral replication. In our results, we suggested that a serial measurement of serum CPK activity might be a useful method for throwing more light on the myocardial damage caused by the autoimmune response. We also used a pathological (TUNEL) method to detect apoptotic cells and some apoptotic myocytes in the myocardium in late phase EMC virus-induced myocarditis.

著者関連情報
© 1996 Japanese Association for Laboratory Animal Science
前の記事 次の記事
feedback
Top