Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
Article
Epitope Mapping of Murine Monoclonal Antibodies against Human Immunodeficiency Virus Type 1 Nef
Kaori OTAKEYoichi FUJIIMasato TASHIROAkio ADACHIJunzoh KITOH
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1997 年 46 巻 1 号 p. 53-58

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It has shown that the human immunodeficiency virus type 1 (HIV-1) Nef protein has the high antigenicity in HIV-1 seropositive individuals. We newly obtained seven monoclonal antibodies (mAbs). To identify the antigenic determinants of HIV-1 Nef protein against murine, epitope mapping of the mAbs was performed by enzyme-linked immunosorbent assay (ELISA) by using several recombinant truncated Nef fusion proteins, that were expressed in Esherichia coli, and synthetic peptides. The results showed that mAbs A6, A7, F2, F3, F4, F8 and E5 recognized epitopes on Nef protein located at amino acid residues 18-26, 28-45, 115-137, 128-137, 115-126, 128-137, and 170-181, respectively.

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© 1997 Japanese Association for Laboratory Animal Science
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