Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
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Identification of Rat Serum Alkaline Phosphatase Isoenzyme by means of Wheat Germ Agglutinin
Hiroshi WADANoboru NIWATakashi HAYAKAWAHaruhito TSUGE
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1997 年 46 巻 1 号 p. 89-92

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Wheat germ agglutinin (WGA) precipitates bone type serum alkaline phosphatase (sALP) isoenzyme specifically. The precipitates are composed of the macromolecules of WGA and "bone type sALP" (WGA-ALP complex). In order to use bone type sALP as a marker in polyacrylamide gel electrophoresis (PAGE), a method to separate "bone type sALP" from the "WGA-ALP complex" was established by using N-acetyl-D-glucosamine (GlcNAc)-Sepharose 6B column chromatography. It was concluded that this method is useful for clinical examination in the rat.

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© 1997 Japanese Association for Laboratory Animal Science
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