Immunolocalization of Steroidogenic Enzymes in the Fetal, Neonatal and Adult Testis of the Shiba Goat

Qiang WENG; Mohamed S. MEDAN; LongQuan REN; Gen WATANABE; Toshio TSUBOTA; Kazuyoshi TAYA

doi:10.1538/expanim.54.451

Note

Immunolocalization of Steroidogenic Enzymes in the Fetal, Neonatal and Adult Testis of the Shiba Goat

Qiang WENG, Mohamed S. MEDAN, LongQuan REN, Gen WATANABE, Toshio TSUBOTA, Kazuyoshi TAYA

Author information

Qiang WENG
Faculty of Biological Science and Technology, Beijing Forestry University
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Mohamed S. MEDAN
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University
LongQuan REN
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University
Gen WATANABE
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University
Toshio TSUBOTA
Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University
Laboratory of Zoo and Wildlife Medicine, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
Kazuyoshi TAYA
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University

Keywords: goat testes, immunohistochemistry, steroidogenic enzymes

JOURNAL OPEN ACCESS

2005 Volume 54 Issue 5 Pages 451-454

DOI https://doi.org/10.1538/expanim.54.451

Details

Abstract

The localizations of steroidogenic enzymes (P450scc, 3βHSD, P450c17 and P450arom) in testes of Shiba goats were investigated by immunohistochemistry. P450scc, 3βHSD, P450c17 and P450arom were detected in all Leydig cells of adults. P450scc and P450c17 were observed in most Leydig cells in the fetus (90 days) and neonate (15 days). 3βHSD and P450arom were found in some Leydig cells of the fetus with weak immunostaining but the numbers of immunopositive Leydig cells and intense immunostaining were increased in Leydig cells of the neonate. These results suggest that Shiba goat testes have the ability to synthesize progestin, androgen and estrogen in the fetus, neonate and adult, and synthesis of these steroid hormones showed an age-related rise.

Corresponding author

Register with J-STAGE for free!