Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
Original
Simple generation of hairless mice for in vivo imaging
Yoshikazu HoshinoSeiya MizunoKanako KatoSaori Mizuno-IijimaYoko TanimotoMiyuki IshidaNoriko KajiwaraTomoki SakasaiYoshihiro MiwaSatoru TakahashiKen-ichi YagamiFumihiro Sugiyama
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2017 Volume 66 Issue 4 Pages 437-445

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Abstract

The in vivo imaging of mice makes it possible to analyze disease progress non-invasively through reporter gene expression. As the removal of hair improves the accuracy of in vivo imaging, gene-modified mice with a reporter gene are often crossed with Hos:HR-1 mutant mice homozygous for the spontaneous Hrhr mutation that exhibit a hair loss phenotype. However, it is time consuming to produce mice carrying both the reporter gene and mutant Hrhr gene by mating. In addition, there is a risk that genetic background of the gene-modified mice would be altered by mating. To resolve these issues, we established a simple method to generate hairless mice maintaining the original genetic background by CRISPR technology. First, we constructed the pX330 vector, which targets exon 3 of Hr. This DNA vector (5 ng/µl) was microinjected into the pronuclei of C57BL/6J mice. Induced Hr gene mutations were found in many founders (76.1%) and these mutations were heritable. Next, we performed in vivo imaging using these gene-modified hairless mice. As expected, luminescent objects in their body were detected by in vivo imaging. This study clearly showed that hairless mice could be simply generated by the CRISPR/Cas9 system, and this method may be useful for in vivo imaging studies with various gene-modified mice.

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© 2017 Japanese Association for Laboratory Animal Science
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