固定家兎を使用しての發熱性注射液の檢定方法に關する研究

抄録

Our experiments, were planned in order to discover, simplest method of testing whether a solution which is injected into the rabbit's ear-vein is pyrogenic or not. When a rabbit was fastened with its back its body temperature declined gradually until it reached its lowest point in two hours, and thereafter the temperature curve traced a certain pattern during two hours' observation when the animal was healthy. Results obtained from forty rabbits convinced us that the patterns are normal for healthy animals and that they are reliable from statistical point of view. As standards of temperature measurement the following three criteria were chosen : First, the maximum temperature rise, secondly, the maximum difference of temperature between the beginning and the end of a thirty mimute period among four such periods after injection, and thirdly, the type of temperature curve. Any deviation from the standard indicated the existence of pyrogen. Because of the fact that when some pyrogenic substances were injected into the rabbit's vein two hours after the animal was held in position, the temperature curve showed usually a certain deviation from the standard, this method seemed to us to be probably quite suited for our experimental purpose.