pH電極によるCholinesterase初期反応の測定法について

抄録

Methods for chemical determination of ChE activity are as follows: titration, manometric, microdiffusion etc. Well-estabilished and commonly-used is Ammon's method using Warburg's manometric technique. Although this method is useful to follow proceeding reactions, the measurement evolves during 30-60min starting only 10min after the reaction is initiated by addition of substrate. Initial reaction states thus go unobserved. In this paper, a method for determination of the initial state of ChE activity was newly developed using pH-electrode. ChE activities in rabbit brain and liver and in human blood cells and serum were studied and a comparison with the manometric method was made.