Specific Real-time PCR Detection of Monkfish Ingredients

Abstract

This study was to establish the optimal real-time PCR method for specific identification of puffer fish and monkfish. In this study, we designed the specific primers and probes for fish 12s and monkfish cytochrome oxidase subunit I (COI), and used them for specific identification of fish and monkfish. Our results showed that the specific primers and probes for monkfish COI could specifically identify 3 monkfish species, respectively, from total 42 samples of aquatic products. Moreover, the primers and probes for COI were very sensitive to detect 0.01% Lophius litulon content in the mixed sample of Theragra chalcogramma and Lophius litulon. Therefore, this method is simple, practical, sensitive and specific; is helpful and useful to identify the products of fish and monkfish.