Food Science and Technology Research
Online ISSN : 1881-3984
Print ISSN : 1344-6606
Original papers
Tyrosinase Activity in Antitumor Compounds of Squid Ink
Tetsushi NARAOKAHun-Sik CHUNGHidemitsu UCHISAWAJin-ichi SASAKIHajime MATSUE
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JOURNALS FREE ACCESS

2000 Volume 6 Issue 3 Pages 171-175

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Abstract

Previous studies have demonstrated antitumor activity in squid ink. This study was carried out to clarify the key component of, and the role of tyrosinase in the antitumor activity. The antitumor fraction, which contains illexin-peptidoglycan, soluble melanin and tyrosinase activity, was isolated from the defatted ink using Tris-HCl buffer extraction, DEAE Sephacel and Sephacryl S-300 chromatography. During the isolation procedure, the behavior of the tyrosinase activity exhibited the same pattern as the antitumor activity. The antitumor fraction could be separated by Phenyl Sepharose CL-4B chromatography into three fractions: illexin-peptidoglycan, tyrosinase and the complex of the two. The fraction containing illexin-peptidoglycan and tyrosinase showed the highest activity against the Meth A tumor in BALB/c mice. This suggests that both these components are needed for the antitumor activity of squid ink.

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© 2000 by Japanese Society for Food Science and Technology
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