1986 Volume 28 Issue 1 Pages 38-51
A new technique to evaluate gastric mucosal blood flow (GMBF) in dog by thermal diffusion method was introduced in this report. This method uses a thermal diffusion probe constructed from a peltier stack, which we calibrated against hydrogen clearence technique. The probe was surgically placed on the canine gastric mucosal surface and the stack was activated and temperature difference between two plates was recorded in terms of millivolts. Using this method, we checked changes of GMBF induced by several drugs or procedure and the following results were obtained. 1) The clearence curve obtained by electrochemically ganerated hydrogen gas clearence method is almost semilogarithmic curve. So we can calculated blood flow value by this method. 2) GMBF showed a gradual increase a few minutes after AOC tetrapeptide application intravenously (8μg/kg/hs), whereas by hydrogen clearence method, GMBF was shown no increase. 3) By ligation of celiac axis, GMBF decreased and by infusion of Vasopressin (0.5 unit/kg/hs) it also showed a manifest decrease. After cardiac arrest induced by rapid injection of potassium chloride, the continuous line showed a blood flow decrease and finally took a steady state. 4) In these states the data obtained by thermal diffusion method and hydrogen clearence method showed a good correlation (r=0.95, p<0 .001). In many aspects, further evaluation of the validity of this method was needed. From these results, it is concluded that this method is useful to check the continuous change of GMBF and has the merit that it is not affected by the presence of hydrogen ion.
