Effect of Heparin on Post Heparin Lipoprotein Lipase and Hepatic TG-lipase

Abstract

Purpose and Method: It is well known that post heparin lipoprotein lipase (HLPL) is activated by heparin. However, the role of heparin in HLPL is still in debate. Therefore, using plasma from heparin-injected rabbits, present study was designed to detect how the activity of HLPL changed, in which heparinase in vitro was added to destroy heparin. The effect of heparin degradation product on LPL activity was also studied. And the effect of FFA or glycerol on hepatic TG-lipase was investigated with rat liver perfusion.
Result and Conclusion: By in vitro addition of heparinase to plasma containing HLPL heparin content was reduced to almost same levels as that of control. It is concluded that heparin itself would have no relation but induction mechanism in plasma HLPL activity. The degradation of heparin by the heparinase produced a substance with high LPL activity and low anticoagulant activity. With rat liver perfusion, while glycerol had no effect on lipase activity, linoleic acid seemed to effect lipase activity in perfusates. It would be supposed that FFA could stimulate the release of lipase from liver, that is, hepatic TG-lipase. And increasing FFA would be one of the factors for secretion of hepatic TG-lipase.