Genes & Genetic Systems
Online ISSN : 1880-5779
Print ISSN : 1341-7568
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Identification of individual barley chromosomes based on repetitive sequences: Conservative distribution of Afa-family repetitive sequences on the chromosomes of barley and wheat
Hisashi TsujimotoYasuhiko MukaiKeisuke AkagawaKiyotaka NagakiJunzo FujigakiMaki YamamotoTetsuo Sasakuma
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1997 Volume 72 Issue 5 Pages 303-309


The Afa-family repetitive sequences were isolated from barley (Hordeum vulgare, 2n = 14) and cloned as pHvA14. This sequence distinguished each barley chromosome by in situ hybridization. Double color fluorescence in situ hybridization using pHvA14 and 5S rDNA or HvRT-family sequence (subtelomeric sequence of barley) allocated individual barley chromosomes showing a specific pattern of pHvA14 to chromosome 1H to 7H. As the case of the D genome chromosomes of Aegilops squarrosa and common wheat (Triticum aestivum) hybridized by its Afa-family sequences, the signals of pHvA14 in barley chromosomes tended to appear in the distal regions that do not carry many chromosome band markers. In the telomeric regions these signals always placed in more proximal portions than those of HvRT-family. Based on the distribution patterns of Afa-family sequences in the chromosomes of barley and D genome chromosomes of wheat, we discuss a possible mechanism of amplification of the repetitive sequences during the evolution of Triticeae. In addition, we show here that HvRT-family also could be used to distinguish individual barley chromosomes from the patterns of in situ hybridization.

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© 1997 by The Genetics Society of Japan
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