Genes & Genetic Systems
Online ISSN : 1880-5779
Print ISSN : 1341-7568
Full papers
piggyBac- and phiC31 integrase-mediated transgenesis in Drosophila prolongata
Ayumi KudoTakeshi AwasakiYukio IshikawaTakashi Matsuo
Author information
JOURNALS OPEN ACCESS FULL-TEXT HTML

2017 Volume 92 Issue 6 Pages 277-285

Details
Abstract

The development of transgenesis systems in non-model organisms provides a powerful tool for molecular analysis and contributes to the understanding of phenomena that are not observed in model organisms. Drosophila prolongata is a fruit fly that has unique morphology and behavior not found in other Drosophila species including D. melanogaster. In this study, we developed a phiC31 integrase-mediated transgenesis system for D. prolongata. First, using piggyBac-mediated transgenesis, 37 homozygous attP strains were established. These strains were further transformed with the nosP-Cas9 vector, which was originally designed for phiC31-mediated transgenesis in D. melanogaster. The transformation rate varied from 0% to 3.4%. Nine strains with a high transformation rate of above 2.0% were established, which will serve as host strains in future transformation experiments in D. prolongata. Our results demonstrate that genetic tools developed for D. melanogaster are applicable to D. prolongata with minimal modifications.

Information related to the author
© 2017 by The Genetics Society of Japan
Previous article Next article
feedback
Top