Japanese Journal of Grassland Science
Online ISSN : 2188-6555
Print ISSN : 0447-5933
ISSN-L : 0447-5933
Pedigree Assessment Using Random Amplified Polymorphic DNA Markers in Maize (Zea mays L.) Inbred Lines
Motoshige KAWATASeiji YAZAKIYoshihide MONMAYasuo MIURAFumio TAKAIWAYoshiya SHIMAMOTOSeiichi UETA
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JOURNAL OPEN ACCESS

1995 Volume 41 Issue 3 Pages 251-255

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Abstract
In this paper, pedigree assessment in 24 maize inbred lines was performed using the DNA markers generated by RAPD (random amplified polymorphic DNAs). 128 fragments generated by 32 primers of arbitary sequence were used to assess the level of DNA variation. Identification of 24 maize inbred lines was carried out by polymorphic DNAs generated from two primers. It was revealed that it was possible to assess DNA polymorphisms using RAPD without any information of DNA sequences. From the data of cluster analysis, 24 inbred lines were divided into six groups. It was suggested that there were long genetic distances between dent inbred lines and flint ones. It was supposed that inbred lines used in this study were derived from different origins. Polymorphic band characteristic to flint inbred lines was also observed. It was suggested that analysis of the functional gene may be possible from the results of our study.
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