In order to find out whether M. lepraemurium grown on egg-yolk solid medium would be able to grow in the Nakamura's liquid medium or not, the bacterial suspensions of M. lepraemurium grown on solid medium were inoculated into various synthetic liquid media which had been established by Nakamura, and the growth was measured by a slide culture and bacillary counting methods.
The results obtained demonstrate that there are no growth of M. lepraemurium grown on Ogawa's solid medium, in liquid media.
On the contrary, unequivocal multiplication of M. lepraemurium obtained from mouse leproma in NC-5 as well as ND-5 medium was indicated.
At present, it is uncertain whether the fact might depend upon the qualitative difference between M. lepraemurium in vitro and in vivo, or quantitative contents of growth potentiality of bacilli present in the starting material.