ウサギ赤血球ロゼット形成法で調べたモルモット細胞性免疫におよぼすリファンピシン(RFP)の免疫抑制効果

儀同 政一; 堤 貞衛

doi:10.5025/hansen1977.50.55

儀同政一, 堤貞衛

著者情報

ジャーナルフリー

50 巻 (1981) 2 号 p. 55-61

DOI https://doi.org/10.5025/hansen1977.50.55

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公開日
公開日: 2008/02/26 [早期公開] 公開日: - 受付日: 1980/10/17 改訂日: - 受理日: -
訂正情報
訂正日: 2008/02/26 訂正理由: - 訂正箇所: 論文受付日訂正内容: 訂正後 : 19801017

訂正日: 2008/02/26 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : The immunodepressive effect of RFP was examined by a method of rosette formation between guinea pig lymphocyte (GPL) and rabbit red blood cells (RRBC). The oral dose of 30mg/kg of RFP once every day for 12 days brought a serious immunodepression to the guinea pigs whose CMI was recovered during the term of above 2 months after thymectomy (named recovered guinea pigs) whereas it could not be detected in the guinea pigs whose CMI was still depressed due to the insufficient term after thymectomy (named immunodepressed ones). However, the depressive effect by the dose of 10mg/kg of RFP could not be detected. On the other hand, the influence by the dose of 30mg/kg of Sulfadimethoxine (SD) could not be detected either by the use of recovered guinea pigs or by immunodepressed ones. Since the dosage of DDS (20mg/kg) examined in the preceding report(⁸) about its immunodepressive effect on normal and recovered guinea pigs was too high to consider the effect on patients under DDS therapy, the influence of 5mg/kg of DDS on recovered guinea pigs was examined. However, in spite of positive result by Ghei, et al.(¹³) who noticed the immunodepression in volunteers by lymphocyte transformation method, the influence of DDS at the dosage of 5mg/kg could not be clearly noticed by this method. Along with these results, the discussions were made reference of the usage of this method, the attention to RFP and DDS therapies and the difference between DDS and SD. References
Right : The immunodepressive effect of RFP was examined by a method of rosette formation between guinea pig lymphocyte (GPL) and rabbit red blood cells (RRBC). The oral dose of 30mg/kg of RFP once every day for 12 days brought a serious immunodepression to the guinea pigs whose CMI was recovered during the term of above 2 months after thymectomy (named recovered guinea pigs) whereas it could not be detected in the guinea pigs whose CMI was still depressed due to the insufficient term after thymectomy (named immunodepressed ones). However, the depressive effect by the dose of 10mg/kg of RFP could not be detected. On the other hand, the influence by the dose of 30mg/kg of Sulfadimethoxine (SD) could not be detected either by the use of recovered guinea pigs or by immunodepressed ones. Since the dosage of DDS (20mg/kg) examined in the preceding report(⁸) about its immunodepressive effect on normal and recovered guinea pigs was too high to consider the effect on patients under DDS therapy, the influence of 5mg/kg of DDS on recovered guinea pigs was examined. However, in spite of positive result by Ghei, et al.(¹³) who noticed the immunodepression in volunteers by lymphocyte transformation method, the influence of DDS at the dosage of 5mg/kg could not be clearly noticed by this method. Along with these results, the discussions were made reference of the usage of this method, the attention to RFP and DDS therapies and the difference between DDS and SD.

訂正日: 2008/02/26 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/02/26 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

30mg/kgのRFPを1日1回12日間にわたり,胸せん摘出後2,月間以上経て免疫回復したモルモット(回復モルモット)へ経口投与し,標記方法で投与直前直後を調べたところ著明な免疫低下を認めたが10mg/kgでは低下を認めずまた先報の20mg/kg DDSの効果に較べ30mg/kgでも低下はやや少なかった。また胸腺摘出後6日から使用のモルモット(抑制モルモット)では30mg/kgでも一層の低下は認めなかった。30mg/kgスルファジメトキシン(SD)は回復,抑制両モルモットに関しロゼット%低下をまったく生ぜずDDSとの著しい相違を認めた。しかしDDSも5mg/kgでは標記方法で低下を検出できず,リンパ球幼若化法でヒトへの2mg/kgDDS投与による低下を検出したGheiらの報告に照らし,DDSの細胞核への作用の可能性や細胞核作用物質の場合特にリンパ球幼若化検査は標記方法より感度が高いのではないかという可能性にも言及した。討論として,標記方法は簡便かつじん速検査として利点があり,モルモット免疫の胸腺摘出後の免疫回復性を利用することで回復モルモットでは免疫抑制作用を,抑制期では同賦活作用を調べうること,胸腺摘出動物を用いることで正常モルモットによる原法応用より著明に感度が上昇したこと,本報結果で低投与量では免疫低下を検出しなかったとはいえ特にDDSの場合長期使用時の蓄積がもたらす免疫低下作用の危険性などに言及した。

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