癩腫より分離、培養された抗酸菌HI-75のゲノム解析 16SリボゾームRNAのダイレクトシークエンシング

阪井 哲男; 松尾 英一; 脇坂 晟

doi:10.5025/hansen.65.180

癩腫より分離、培養された抗酸菌HI-75のゲノム解析

16SリボゾームRNAのダイレクトシークエンシング

阪井哲男, 松尾英一, 脇坂晟

著者情報

キーワード: 抗酸菌HI-75, DNAシークエンス, 16SリボゾームRNA, Leprosy(ハンセン病)

ジャーナルフリー

1996 年 65 巻 3 号 p. 180-185

DOI https://doi.org/10.5025/hansen.65.180

詳細

発行日: 1996/11/30 受付日: 1996/09/09 J-STAGE公開日: 2008/02/26 受理日: 1996/10/17 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/02/26 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) Skinsnes OK, Matsuo E, Chang PHC, Anderson B. In vitro cultivation of leprosy bacilli in hyaluronic acid based medium. 1. preliminary report. Int J Lepr 43: 193-203 (1975).
2) Sasaki K. On the mice footpads inoculation of a leproma-derived and cultivable mycobacterium. The Annual Report of National Institute for Leprosy Research. 3lth Issue, National Institute for Leprosy Research pp, 54-55 Tokyo, 1985 (in Japanese).
3) Edwards U, Rogall T, Bloker H, Emde M, Bottger EC. Isolation and direct complete nucleotide determination of entire genes. 184 Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17: 7843-7853 (1989).
4) Rogall T, Flohr T, Bottger EC. Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J Gen Microbiol 136: 1915-1920 (1990).
5) Rogall T, Wolters J, Flohr T, Bottger EC. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol 40: 323-330 (1990).
6) Nakamura M. Mycobacterium leprae and M. lepraemurium. Tokai Univ. Press, 1985 (in Japanese).
7) Matsuo E, Komatsu A, Maekawa S, Furuno Y, Matsushita A, Sumiishi A, Sasaki N, Skinsnes OK. On the beta-glucuronidase protein (BGBP) of microorganism. Its purification, the antiserum preparation against that and its localization in leproma and the other infectious lesions shown by immunihistologic method. Jpn J Leprosy 64: 35-46 (1994).
8) Stanford JL, Bird RG, Carswell JW, Draper P, Lowe C, McDougall AC, Mclntyre G, Patty SR, Rees RJW. A study of alleged leprosy bacilli strain HI-75. Int J Lepr 45: 101-106 (1977).
9) Hamit S. An experimental nerve lesion simulating leprous neuropathy produced in the nude mice by the inoculation of a leproma-derived and cultivable Mycobacterium HI-75. Jpn J Lepr (1996) (in press)
10) Brosius J, Palmer ML, Kennedy PL, Noller HF. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75: 4801-5 (1978). Jpn. J. Leprosy 65, 180-185 (1996)

Right : 1) Skinsnes OK, Matsuo E, Chang PHC, Anderson B. In vitro cultivation of leprosy bacilli in hyaluronic acid based medium. 1. preliminary report. Int J Lepr 43: 193-203 (1975).
2) Sasaki K. On the mice footpads inoculation of a leproma-derived and cultivable mycobacterium. The Annual Report of National Institute for Leprosy Research. 3lth Issue, National Institute for Leprosy Research pp, 54-55 Tokyo, 1985 (in Japanese).
3) Edwards U, Rogall T, Bloker H, Emde M, Bottger EC. Isolation and direct complete nucleotide determination of entire genes. 184 Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17: 7843-7853 (1989).
4) Rogall T, Flohr T, Bottger EC. Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J Gen Microbiol 136: 1915-1920 (1990).
5) Rogall T, Wolters J, Flohr T, Bottger EC. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol 40: 323-330 (1990).
6) Nakamura M. Mycobacterium leprae and M. lepraemurium. Tokai Univ. Press, 1985 (in Japanese).
7) Matsuo E, Komatsu A, Maekawa S, Furuno Y, Matsushita A, Sumiishi A, Sasaki N, Skinsnes OK. On the beta-glucuronidase protein (BGBP) of microorganism. Its purification, the antiserum preparation against that and its localization in leproma and the other infectious lesions shown by immunihistologic method. Jpn J Leprosy 64: 35-46 (1994).
8) Stanford JL, Bird RG, Carswell JW, Draper P, Lowe C, McDougall AC, Mclntyre G, Patty SR, Rees RJW. A study of alleged leprosy bacilli strain HI-75. Int J Lepr 45: 101-106 (1977).
9) Hamit S. An experimental nerve lesion simulating leprous neuropathy produced in the nude mice by the inoculation of a leproma-derived and cultivable Mycobacterium HI-75. Jpn J Lepr (1996) (in press)
10) Brosius J, Palmer ML, Kennedy PL, Noller HF. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75: 4801-5 (1978).

訂正日: 2008/02/26 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/02/26 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

Skinsnesらにより,1975年に,癩腫より分離され,その後培養を続けた抗酸菌M. HI-75の種の同定の目的で,16SリボゾームRNAをRT-PCRにて増幅し,DNAのダイレクトシークエンシングを行った。細菌の種特異的な可変領域を含む973塩基の配列を,M. HI-75のシーケンスと,Gen-Bankその他のData base上の他の抗酸菌と比較検討した。M. HI-75は5塩基,即ち0.5%の相違をもって最もM. scrofulaceumに近似していた。
一方,佐々木らによれば,M. HI-75はヌードマウス等の末梢神経に侵潤する。この性質はM.lepraeに特徴的であるとされている。よって今回の検索の結果,M. HI-75は癩腫の原因菌のひとつでありえるのか,またはM. scrofulaceumも末梢神経病変を起こすのかを検索する必要性が示唆された。

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