Health and Behavior Sciences
Online ISSN : 2434-7132
Print ISSN : 1348-0898
Changes of interstitial free mononuclear cells in the process of disuse muscle atrophy
Miki SakamotoAzusa MaeharaMasuo MuroKazuyuki Oka
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2012 Volume 10 Issue 2 Pages 93-100

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Abstract

  Introduction: It is known that skeletal muscle with disuse atrophy exhibits reduction of muscle mass, diminution of fiber cross-sectional area (FCSA), etc. In the process of disuse atrophy, it is also known that the number of leukocytes and the frequency of cell division of non-endothelial cells are increased in the interstitium. We investigated the condition of disuse atrophy and the numerical changes of interstitial free mononuclear cells with the passage of time after immobilization.

  Materials and methods: The soleus muscles of ICR mice were used in this study. In the immobilized groups, the left hindlimb was immobilized at the ankle joint at maximum planter flexion. After routine ATPase staining, FCSA of each muscle fiber (type I, type II) was measured separately. The number of interstitial free mononuclear cells per unit area (500 × 500 µm) was counted by using longitudinal paraffin sections stained by hematoxylin-eosin and it was defined as the interstitial free mononuclear cell index.

  Results: FCSA of both type I and type II fibers were smaller in each immobilized group compared to the control group. The interstitial free mononuclear cell index tended to show a rapid increase after 3 days of immobilization and the index tended to be continuously higher compared with the control group after day 3.

  Discussion: By comparing FCSA and the interstitial free mononuclear cell index, it was speculated that the state of muscle disuse atrophy can be evaluated easily and promptly using the interstitial free mononuclear cell index.

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© 2012 Health and Behavior Sciences
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