2021 年 71 巻 2-4 号 p. 101-107
Background
Aortic valve stenosis (AVS) is the most common heart valve disease in elderly society. The effective treatment
for AVS is surgical aortic valve replacement but with extremely invasive. Although transcatheter aortic valve
replacement also is performed for highly risk patients with minimally invasive, it has to resolve the problem of
bioprosthetic valve durability in future. However, there is no effective medical treatment established for AVS to
inhibit acceleration of aortic valve calcification.
Objectives
We aimed to confirm whether the ascending aorta is the best location for AVS to innovate local and selective medical
therapy or not.
Methods and Results
After anesthetized by isoflurane, the wild type rat was stablished on operating table. Opening chest to heart by
median sternotomy approach, the catheter was inserted into left ventricular from apical for body perfusion. We
injected saturated solution of amido black 10B (AB) into ascending aorta above sino-tubular junction about 0.5 cm
from aortic valves without aortic dissection observed, and body perfusion was performed by saline containing heparin
in order to prevent embolism happened in vascular. After 30 minutes, the heart with the ascending aorta was
extracted. We used optical microscope to check the existence of AB at around of aortic valves or not. Interestingly,
AB was detected at around of aortic valves and valsalva sinus of the aorta and myocardium in all rats.
Conclusions
These results suggested that AB injected is delivered from the ascending aorta to aortic valves through vasa
vasorum. These data provide very important evidences for local medical therapy development for AVS patients.