Synthesis of Carboxylic Group Displayed Water-Dispersible Metal Oxide Nanoparticles toward Fabrication of Protein Fused Nanoparticle for Biomedical Application

Abstract

In this study, we have synthesized of COOH group displayed metal oxide, Fe₃O₄ and CeO₂ nanoparticles via thermal decomposition of 3,4-dihydroxyhydroxycinnamic acid (DHCA)-metal complex in high temperature and pressure water. The COOH groups useful for binding the protein and nanoparticles were displayed on the synthesized metal oxide nanoparticles. In addition, the synthesized particles have narrow size distribution and water dispersibility. Cytokines such as IL-12 and TNF-a were not produced from the dendritic cells of mice by co-incubation with our synthesized nanoparticles. This indicates that the synthesized nanoparticles had no immune stimulating property for the dendritic cells of the mouse.