DNA Polymorphism of Chloroplast SSR Regions in Various Vegetables and its Application to Analyses of Interspecific Polymorphisms in Allium species

Abstract

Polymorphic analyses of simple sequence repeat regions on chloroplast genomes in 34 vegetables were conducted using a set of consensus SSR primer pairs. A sufficient number of DNA markers were obtained in this study and could be available for polymorphic analyses at the species level. The most successful result was obtained from the polymorphic analyses of cultivated and wild species in Allium. The DNA fingerprints of Allium facilitated the identification of each species except in one difficult case. The fingerprint of a natural hybrid, A. × wakegi, could not be distinguished from that of its estimated seed parent, A. fistulosum. The DNA markers obtained from this study seem to have potential not only for breeding purposes but also for cultivar identification in various species of vegetables.