Temperature Effect on the Separation Performance of Chromatography

抄録

The effect of temperature T on the chromatographic separation performance was examined by using a model separation system: polyphenol isocratic elution separation by polymer-resin chromatography with ethanol-water mobile phases within the temperature range 15-45°C. HETP decreased with increasing T. The reduced HETP and the reduced velocity plots can be approximated by a single universal curve. The equations describing the distribution coefficient K as a function of T were also developed. A simple equation was derived, which can describe the resolution of two components R_s as a function of T. R_s did not increase significantly with T while the separation time can be reduced.