３次元ナノプロトタイピング法による多検体DNA解析チップの開発

抄録

The importance of the micro fluidics systems for high throughput post-genome analysis has increasing more and more. We proposed and fabricated an integrated 6 microchip capillary electrophoresis units in a small area of 30mm×30mm for high throughput DNA analysis. The design of the structure was performed using fluid dynamics of electrophoresis simulation to improve DNA separation resolution. As a result, narrow width of micro channel at the corner was adapted. As for the simple fabrication method of the microchip, we developed the PMMA structure of micro fluid channel using lithography, molding and fusion bonding techniques. It was demonstrated that the proposed integrated structure of micro channels results in a good performance of the on-chip DNA separation in a small one unit area of 9mm×9mm.