In this study, we propose an electrochemiluminescence (ECL)-induced fluorescence detection chip toward portable point-of-care diagnostics. A prototype chip consists of polydimethylsiloxane (PDMS)-based flow channels for target samples and on-demand multi-color microfluidic excitation sources. The microfluidic ECL cells were fabricated via a heterogeneous bonding technique through the use of self-assembled monolayers. The flow channels for target samples, which were formed by soft lithography, were combined with the excitation source. A pink fluorescence of resorufin was successfully detected with yellow ECL emission of 5,6,11,12-tetraphenyltetracene (rubrene). The proposed principle can open a new possibility for on-onsite multiple fluorescence detections.