Assay of Heparin in Plasma Using a Chromogenic Substrate and Its Clinical Applications

Hiroshi HASEGAWA; Yutaka OGUMA; Hideaki TAKEI; Tsukasa SEYA; Masao YAMAUCHI; Toshio MURAKOSHI; Hiroshi NAGATA; Makoto MURAO

doi:10.1536/ihj.21.367

Hiroshi HASEGAWA, Yutaka OGUMA, Hideaki TAKEI, Tsukasa SEYA, Masao YAMAUCHI, Toshio MURAKOSHI, Hiroshi NAGATA, Makoto MURAO

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Hiroshi HASEGAWA
First Department of Internal Medicine, Hokkaido University School of Medicine
Yutaka OGUMA
First Department of Internal Medicine, Hokkaido University School of Medicine
Hideaki TAKEI
First Department of Internal Medicine, Hokkaido University School of Medicine
Tsukasa SEYA
First Department of Internal Medicine, Hokkaido University School of Medicine
Masao YAMAUCHI
First Department of Internal Medicine, Hokkaido University School of Medicine
Toshio MURAKOSHI
First Department of Internal Medicine, Hokkaido University School of Medicine
Hiroshi NAGATA
First Department of Internal Medicine, Hokkaido University School of Medicine
Makoto MURAO
First Department of Internal Medicine, Hokkaido University School of Medicine

Keywords: Heparin, Chromogenic substrate, Activated partial thromboplastin time, Antithrombin III, Fibrinopeptide A, Disseminated intravascular coagulation

JOURNAL FREE ACCESS

1980 Volume 21 Issue 3 Pages 367-380

DOI https://doi.org/10.1536/ihj.21.367

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Abstract

The concentration of heparin in plasma was measured using a chromogenic substrate. It appeared that the measurement of heparin concentration in plasma was important in therapeutic control and evaluation of heparin. There was a correlation between the activated partial thromboplastin time (APTT) and heparin concentration in plasma, but since the gradients of regression line differed in each of the cases, measurement of APTT alone would give a different estimate of heparin concentration in each case. Fibrinopeptide A (FPA) is considered as the best indicator for evaluation of therapeutic effects of heparin. Therapeutic heparin concentrations were defined as ranging from 0.2 to 1.2IU/ml because the normalization of FPA was observed and there happened no hemorrhagic accidents in that range.

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