2017 Volume 15 Issue 3-4 Pages 67-73
Secretory granules (SGs) of salivary glands are considered to be generated as an immature granule and to mature by condensing their contents. Syntaxin 6 and vesicle associated membrane protein 4 (VAMP4) are detected in the membrane fractions of immature but not mature SGs, suggesting that syntaxin 6 and VAMP4 are transported from SGs to other organelles during the process of granule maturation. To study the role of syntaxin 6 in the biogenesis of granules, we transfected syntaxin 6 fused with enhanced green fluorescent protein(EGFP)into primary cultured parotid acinar cells. The EGFPsyntaxin 6 signal overlapped with that of an early endosome marker, early endosome antigen 1(EEA1), which was detected using immunofluorescence. The localization of EGFP-fused VAMP4 was also similar to that of EEA1, suggesting that syntaxin 6 and VAMP4 are transported from immature SGs to early endosomes. Transfection of a syntaxin 6 mutant that lacks a SNARE-binding domain caused a decrease in the number of SGs although the localization of the mutant was unchanged from that of the wild type syntaxin 6. These results suggest that syntaxin 6 has an important role in the maintenance of SGs in parotid acinar cells.