A METHOD FOR SEPARATION AND DETERMINATION OF URINARY ADRENALINE AND NORADRENALINE

Abstract

Adrenaline and noradrenaline in alumina extract of human urine were measured with an ion exchange chromatography and an automated fluorometric determination. A few ng of adrenaline was detected by this method. The recoveries of adrenaline and noradrenaline added to alumina extract of urine were 83 and 96%, respectively. It was shown that some compounds in alumina extract of urine interfere with the differential fluorometric, method for the determination of catecholamines widely used, but there was recognized almost no interference in this chromatographic method. Therefore, the present method is considered to give more accurate values of urinary adrenaline and noradrenaline than the differential fluorometric method.