ANALYSIS OF CATECHOLAMINES BY HIGH-SPEED LIQUID CHROMATOGRAPHY

Abstract

Rapid separation of dopa, noradrenaline, octopamine, adrenaline and dopamine was accomplished by means of high speed liquid chromatography with gradient elution of SCX column by Na⁺-containing aqueous solution. After this chromatographic separation, adrenaline and noradrenaline were fluorometrically determined by an automated trihydoxyindole method and dopamine was measured by an automated ethylenediamine method. By the present procedure 1 ng of adrenaline and noradrena-line and 10 ng of dopamine were easily detected. And, the former two amines in urine-alumina extract can be analyzed very rapidly such as 3 samples per hour.