STUDIES ON LYSOZYME IV

CHEMICAL MODIFICATION OF LYSOZYME WITH 1, 2- CYCLOHEXANEDIONE

Abstract

Arginine was reacted with 1, 2-cyclohexanedione even under a mild condition. This reaction has been used for studying the relationship between the arginyl residues and the biological activity of hen egg white lysozyme. The seven of all the eleven arginyl residues of lysozyme were specifically modified with 1.2-cyclohexanedione at the weak alkaline pH for two hours. The modification did not affect against the hydrolytic activity toward glycol chitin, but abolished the lytic activity toward Micro-coccs lysodeikticus cells.