The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification

Shuhei Moriguchi; Masahiro Abe; Muneyoshi Kimura; Chiemi Yoshino; Masaru Baba; Chikako Okada; Koji Izutsu; Shuichi Taniguchi; Hideki Araoka; Akiko Yoneyama

doi:10.2169/internalmedicine.9810-17

CASE REPORTS

The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification

Shuhei Moriguchi, Masahiro Abe, Muneyoshi Kimura, Chiemi Yoshino, Masaru Baba, Chikako Okada, Koji Izutsu, Shuichi Taniguchi, Hideki Araoka, Akiko Yoneyama

Author information

Shuhei Moriguchi
Department of Infectious Diseases, Toranomon Hospital, Japan
Masahiro Abe
Department of Infectious Diseases, Toranomon Hospital, Japan
Muneyoshi Kimura
Department of Infectious Diseases, Toranomon Hospital, Japan
Chiemi Yoshino
Department of Infectious Diseases, Toranomon Hospital, Japan
Masaru Baba
Department of Infectious Diseases, Toranomon Hospital, Japan
Chikako Okada
Department of Infectious Diseases, Toranomon Hospital, Japan
Koji Izutsu
Department of Hematology, Toranomon Hospital, Japan
Shuichi Taniguchi
Department of Hematology, Toranomon Hospital, Japan
Hideki Araoka
Department of Infectious Diseases, Toranomon Hospital, Japan
Akiko Yoneyama
Department of Infectious Diseases, Toranomon Hospital, Japan

Keywords: Legionella pneumonia, Legionella pneumophila serogroup 5, loop-mediated isothermal amplification, hematological malignancy, malignant lymphoma

JOURNAL OPEN ACCESS

2018 Volume 57 Issue 7 Pages 1045-1048

DOI https://doi.org/10.2169/internalmedicine.9810-17

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Abstract

A 60-year-old man developed pneumonia after undergoing autologous peripheral blood stem cell transplantation for diffuse large-B cell lymphoma. A urinary antigen test and sputum culture were both negative for Legionella pneumophila; however, a sputum sample that was examined by loop-mediated isothermal amplification (LAMP) was positive for Legionella spp. On admission, the results of blood culturing using a BACTEC system were negative for 7 days. However, L. pneumophila serogroup 5 was detected in a blood subculture using WYOα medium. The patient was successfully treated with a fluoroquinolone-based regimen. LAMP is useful for the diagnosis of Legionella spp.

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