Evaluation of Sandwich ELISA Galactomannan Test in Samples of Positive LA Test and Positive Aspergillus Antibody

Shigefumi MAESAKI; Sumio KAWAMURA; Kohji HASHIGUCHI; Mohammad Ashraf HOSSAIN; Eisuke SASAKI; Yoshitsugu MIYAZAKI; Kazunori TOMONO; Takayoshi TASHIRO; Shigeru KOHNO

doi:10.2169/internalmedicine.38.948

Shigefumi MAESAKI, Sumio KAWAMURA, Kohji HASHIGUCHI, Mohammad Ashraf HOSSAIN, Eisuke SASAKI, Yoshitsugu MIYAZAKI, Kazunori TOMONO, Takayoshi TASHIRO, Shigeru KOHNO

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Shigefumi MAESAKI
The Second Department of Internal Medicine, Nagasaki University School of Medicine
Sumio KAWAMURA
The Second Department of Internal Medicine, Nagasaki University School of Medicine
Kohji HASHIGUCHI
The Second Department of Internal Medicine, Nagasaki University School of Medicine
Mohammad Ashraf HOSSAIN
The Second Department of Internal Medicine, Nagasaki University School of Medicine
Eisuke SASAKI
The Second Department of Internal Medicine, Nagasaki University School of Medicine
Yoshitsugu MIYAZAKI
The Second Department of Internal Medicine, Nagasaki University School of Medicine
Kazunori TOMONO
The Second Department of Internal Medicine, Nagasaki University School of Medicine
Takayoshi TASHIRO
The Second Department of Internal Medicine, Nagasaki University School of Medicine
Shigeru KOHNO
The Second Department of Internal Medicine, Nagasaki University School of Medicine

Keywords: aspergillosis, serodiagnosis, aspergilloma, anti-Aspergillus antibody, invasive aspergillosis

JOURNAL FREE ACCESS

1999 Volume 38 Issue 12 Pages 948-950

DOI https://doi.org/10.2169/internalmedicine.38.948

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Abstract

Objective The detection of circulating Aspergillus galactomannan antigen is a useful tool for serodiagnosis of aspergillosis. However, the latex agglutination test for the detection of galactomannan is not completely reliable due to it's low sensitivity. The sandwich ELISA was developed to achieve high sensitivity. Materials The sandwich immunocapture ELISA was evaluated by testing 56 sero-positive and 56 sero-negative samples of circulating galactomannan detected by LA test retrospectively. Results Sixty of the samples were positive for galactomannan as measured by sandwich ELISA. Fifteen samples out of 56 samples negative by LA test were positive by ELISA and 4 samples out of 56 samples positive by LA test were negative by ELISA. Among 47 serum samples positive for anti-Aspergillus antibody, 14 samples were positive by ELISA. Conclusion In conclusion, galactomannan may be detected in more samples of by the new sandwich ELISA than by LA test.
(Internal Medicine 38: 948-950, 1999)

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