血液培養法に關する二, 三の檢討

抄録

I have studied on the routine blood culture method. For this purposes, three strains of Strept. viridans and one strain of Strept. anaerobicus, isolated from endocarditis lenta, and as control Strept. hemolyticus (Cook), and Staphyl. aureus (Terashima), were used. The conclusion gained was as follows.
1) As anticoagulant, one fifth or one tenth volume of 5-10% Na. citrate solution should be added to blood.
2) As enriching media, bouillon, 1% glucose-bouillon, 10% peptone-bouillon and 10% peptone bouillon with vitamine Bl (5 mg) are excellent. When simultaneously two or more media are employed, a better result is obtained than by a single medium. Para-aminoben-zoic acid, thioglycollate natrium and saponin showed no remarkable results.
3) As the media for pour plate cultivation, 1% glucose-agar or normal agar should be used.
4) The blood culture method should be repeated by the direct pour plate cultivation and should use various methods conjointly.