Fluorescent band pattern of chromosomes in Ephedra americana var. andina, Ephedraceae

Masahiro Hizume; Kazuo Tominaga

doi:10.3199/iscb.11.27

Regular Article

Fluorescent band pattern of chromosomes in Ephedra americana var. andina, Ephedraceae

Masahiro Hizume , Kazuo Tominaga

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Keywords: Chromomycin A₃, Chromosome, DAPI, Ephedra americana, Fluorescent banding, Karyotype, Satellite

JOURNAL FREE ACCESS

2016 Volume 11 Issue 2 Pages 27-30

DOI https://doi.org/10.3199/iscb.11.27

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Abstract

Somatic chromosome number of Ephedra americana var. andina was 2n = 14 and its karyotype was composed of ten long metacentric chromosomes and four short subtelocentric chromosomes. Chromomycin A₃ (CMA) bands appeared on small satellites of six metacentric chromosomes indicating CG-rich nucleolar organizer or rRNA locus. Satellites were DAPI-negative and weak DAPI-dots were detected in the interstitial regions of four metacentric chromosomes.

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