2007 Volume 1 Issue 1 Pages 94-96
In order to develop an efficient method for the evaluation of the trans-polyisoprene gene functions at the early stage of transgenic plant growth, in this study, we established a rapid root culture system from plantlets and estimated the quantity of EU rubber from cultured roots under different culture conditions. The results revealed that there was no significant difference between the root cultures grown under a photoperiod (16-h light and 8-h dark/day) and those grown under dark conditions with regard to polyisoprene content and molecular weight distribution. It is clear that the production of polyisoprene is not influenced by the light conditions under which the E. ulmoides root culture is grown. Thus, the root culture system can be used to evaluate polyisoprene biosynthesis and its quality in transgenic plants under different conditions.
