Candida albicans Sterilization Using a Light-Emitting Diode and Methylene Blue

抄録

Background and aims: Conventional fungal treatment using external medication requires considerable time and effort. We herein examined the basic effect of methylene blue (MB) with sodium bicarbonate (NaHCO₃) on Candida albicans sterilization using photodynamic antimicrobial chemotherapy (PACT).
Materials and methods: NaHCO₃ was added to MB to establish a basic pH. Then, C. albicans was smeared on a medium with basic MB followed by irradiation using a red light-emitting diode (LED) with a wavelength of 660 nm. The applied energy fluencies were 5, 10, 15, and 20 J/cm². After 48 h of culture, the effects of the intervention were determined according to the fungal growth area.
Results: The basic effect appeared at a pH range of 8.6 to 8.9 and at 10 and 15 J/cm², while the NaHCO₃ concentration was between 1% and 2%.
Conclusions: Our results suggested that PACT using basic MB was effective for C. albicans sterilization.