We compared the visual fatigue experienced during viewing on a 2D and a 3D television. Twenty participants were individually shown an 80-min clip of the motion picture Avatar on a 2D and a 3D television. Before and after each viewing, the participants completed a questionnaire assessing visual fatigue and discomfort. The visual acuity, accommodation speed and heterophoria of each participant were measured on another day. The results show that the 3D television caused greater visual fatigue than when the 2D television was viewed. Substantial individual differences in visual fatigue were found between participants in the 3D case and these differences varied with the accommodation speed for the non-dominant eye, the difference in visual acuity between eyes and the amount of horizontal heterophoria. Relationships between visual functions and visual fatigue during movie viewing on 3D televisions are discussed.