Improvement for cell extraction and tracking in cell image sequences

Abstract

We have proposed an extraction and tracking technique for image sequence of living cells, and realized a cell image analysis system. However, the efficiency and accuracy of the technique drastically vary depending of the image acquisition conditions. To alleviate the problem, this paper introduces all-in-focus images and automatic threshold determination. The experimental results given in this paper illustrate that the proposed technique improves the efficiency and accuracy in the extraction and tracking process over the previous technique.