Abstract
We studied the influence of the concentration of HES on pulmonary edema during preservation and after reperfusion. The lungs of 25 mongrel dogs were isolated and flushed with 500 ml of one four different solutions at 4°C ventilating with room air. The lungs of each group were flushed and cold-stored as follows : group 1 with modified-Collins (CM) solution (n=6); group 2, CM + 3 % HES (n = 6); group 3, CM + 6 % HES (n = 8); group 4, CM + 9 % HES (n 5). After cold-storage for 24 hours, the isolated left lung was reperfused for 120 min. The left lung was ventilated with room air at a tidal volume of 20 ml/kg of body weight and a rate of 10 cycles/min. The flow rate was maintained at 10 ml/kg/min. The extravascular lung water was calculated and tissue protein present in the lungs was measured, and the histological findings of the lung specimen were examined before and after reperfusion.
The extravascular lung water after reperfusion for 2 hours ; group 1, 88.9 ± 2.1 % ; group 2, 86.6 ± 2.3 % ; group 3, 84.6 ± 1.7 %, ; group 4, 85.6 ± 2. 4 % ; the values in groups 3 and 4 were significantly lower than that in group 1. Tissue protein after perfusion for 2 hours in groups 3 and 4 was significantly lower than that in groups 1 and 2. Pulmonary edema after perfusion was more minimal in group 4 than in the other groups, suggesting that hyperoncotic solution prevents pulmonary edema during both preservation and reperfusion periods.
