日本法科学技術学会誌
Online ISSN : 1881-4689
Print ISSN : 1880-1323
ISSN-L : 1880-1323
DNAによる植物および菌類の種属識別と法科学的応用
轡田 行信
著者情報
ジャーナル フリー 早期公開
電子付録

論文ID: 814

詳細
抄録

 Species identification of botanical and fungal evidences offer beneficial information in crime investigation. As these biological species are highly diverse and complex, their DNA sequence analysis is effective in the field of forensic science. In this study, the internal transcribed spacer (ITS) region on the ribosomal DNA of plant and fungal samples was examined as a targeting locus. For 30 plant and 9 fungal samples, direct sequencing was performed for both ITS1 and ITS2 region using modified universal primers and M13 tag primers. Each resulting sequence was evaluated by a nucleotide BLAST homology search. The top hits for sequence homology against each sample matched the actual species with almost 100% identity. However, homology search does not always result in sequence similarity/identity among closely-related species; therefore, results of homology search must be carefully assessed.

著者関連情報
© 2021 日本法科学技術学会
前の記事 次の記事
feedback
Top