Journal of Applied Glycoscience
Online ISSN : 1880-7291
Print ISSN : 1344-7882
ISSN-L : 1344-7882
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Cyclic Tetrasaccharide in Sake Lees
Hikaru WatanabeMasayuki NakanoKazuyuki OkuHajime AgaTomoyuki NishimotoMichio KubotaShigeharu FukudaMasashi KurimotoYoshio Tsujisaka
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2004 Volume 51 Issue 4 Pages 345-347

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Abstract

We searched for a cyclic tetrasaccharide, cyclo-{→6)-α-D-Glcp-(1→3)-α-D-Glcp-(1→6)-α-D-Glcp-(1→3)-α-D-Glcp-(1→} (abbreviated as CTS) in various foods. GLC analysis revealed that sake lees and sake contained CTS in ranges of 61 to 378 μg/g and 0.23 to 7.05 μg/g, respectively. CTS isolated from sake lees, showed 1H- and 13C-NMR spectra completely identical to those of CTS enzymatically synthesized from starch. We examined CTS formation in the cells of Saccharomyces cerevisiae ATCC9763. Fed with additional sucrose on the 25th day of culture, cells (8.4 g) from a 39-day culture gave a significant amount of CTS (25.2 mg).

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© 2004 by The Japanese Society of Applied Glycoscience
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