Journal of Applied Glycoscience
Online ISSN : 1880-7291
Print ISSN : 1344-7882
ISSN-L : 1344-7882
Regular Papers (in Japanese with English Abstract)
Leaching of Amylose and Amylopectin during Cooking of Rice Grains and Their Effect on Adhesiveness of Cooked Rice
Isao HanashiroKensuke OhtaChieno TakedaHiroyuki MizukamiYasuhito Takeda
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2004 Volume 51 Issue 4 Pages 349-354

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Abstract

Composition and structures of materials leached from rice grains during cooking were investigated in relation to their contribution to the adhesiveness of cooked grains, an important determinant of eating-quality preference. Two cultivars of rice, Koshihikari and Hoshiyutaka, are compared because of their well known sticky and less sticky cooked grains, respectively, and a waxy cultivar Hiyokumochi was also included. The amount of materials leached from rice grains increased with the increase of temperature at which the materials were collected. Hiyokumochi, Koshihikari and Hoshiyutaka yielded leached materials of 0.9, 0.8 and 1.3%, respectively, of rice grains (200 g) cooked at 85°C. More than 93% of the materials were carbohydrate. Fluorescent labeling/GPC showed that the materials comprised two major populations with degree of polymerization (DP) of ∼6000 and ∼1500. The populations were degraded by α-amylase or isoamylase, indicating they were starch-derived α-glucans. Amylose (AM) and amylopectin (AP) fractions were prepared by 1-BuOH precipitation of the leached materials. AM content was 1, 9 and 21% for Hiyokumochi, Koshihikari and Hoshiyutaka, respectively. In comparison with amylose and amylopectin fractionated from starch, both AM and AP had about 1/2 of number-average DP, and chain-length distribution of AP was essentially the same. Replacement of leached liquid of Koshihikari at 85°C to that from Hoshiyutaka resulted in the decrease of adhesiveness of cooked grains of Koshihikari from 334 to 173 erg (average of 40 grains) and the increase of ratio of grains with lower adhesiveness. These results suggested that the higher the ratio of the small amylose leached, the lower the adhesiveness of cooked grains.

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© 2004 by The Japanese Society of Applied Glycoscience
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