Journal of Applied Glycoscience
Online ISSN : 1880-7291
Print ISSN : 1344-7882
ISSN-L : 1344-7882
Large-scale Preparation of 1,2-β-Glucan Using 1,2-β-Oligoglucan Phosphorylase
Koichi AbeMasahiro NakajimaMotomitsu KitaokaHiroyuki ToyoizumiYuta TakahashiNaohisa SugimotoHiroyuki NakaiHayao Taguchi
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JOURNALS FREE ACCESS Advance online publication

Article ID: jag.JAG-2014_011


1,2-β-Glucan was produced enzymatically from 1.0 M sucrose and 0.5 M glucose by the combination of sucrose phosphorylase and 1,2-β-oligoglucan phosphorylase in the presence of 100 mM inorganic phosphate. Accumulation of 1,2-β-glucan in 2 L of the reaction mixture reached over 800 mM (glucose equivalent). Sucrose, glucose and fructose were removed after the reaction by yeast treatment. 1,2-β-Glucan was precipitated with ethanol to obtain 167 g of 1,2-β-glucan from 1 L of the reaction mixture.

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