Abstract
Cyclodextrins (CDs) are used in various fields of application because its ability forming inclusion compounds or complexes with various kinds of substances. Accordingly, recent advances in the research on CD production and application are remarkable. CDs are now commercially produced and the main products are β-CD, CD syrup and powdered CD syrup. And powdered CD syrup (commercial name: Cyclo-TC, Toyoderin) is especially useful for making powdered oily materials with it. Though there are several kinds of special CDs, branched-CDs have not been studied so farr because the production of the special CDs was extremely difficult. Recently, we established a method of producing branched-CDs, in which branched-CDs were prepared by the action of Bacillus m.acerans cyclomaltodextrin glucanotransferase (naacerans enzyme) on branched dextrin of waxy corn starch prepared by removing the CDs with bromobenzene and tetrachloroethane from the macerans enzyme-starch digest. Branched-CDs were effectively produced by the action of macerans enzyme on branched dextrin in the presence of SDS and were further degraded to glucosyl-CDs by the combined action of Aspergillus oryzae a-amylase and glucoamylase. These enzymes also degrade β and γ-CDs, which interfere with the separation of glucosyl-CDs by paper and column chromatography, to glucose. Glucosyl-CDs were separated by paper and column chromatography respectively. Glucosyl-α-CD was crystallized from water and made into a crystal complex with iodine. Glucosyl-CDs have extremely high solubility compared with the original CDs, and solubilize various kinds of oily substances and are extraordinary resistant to the action of Aspergillus oryzae α-amylase. Possible uses of branched-CDs are also discussed in the text.
