2000 Volume 47 Issue 3-4 Pages 281-285
β-Fructofuranosidase from Arthrobacter sp . K-1 was immobilized onto a carrier resin (FE4611) with glutaraldehyde to effectively produce 4G-β-D-galactosylsucrose, which increased Bifidobacterium in the human intestine. Maximum immobilized enzyme was approximately 30 U/g·wet on carrier resin and its immobilized enzyme yield was 76.6%. Optimal pH broadened and optimum temperature increased in response to immobilization. The pH stable range for immobilized enzyme was wider than that for the native enzyme. Both immobilized and native enzymes were stable at temperatures lower than 45°C, whereas the immobilized enzyme was less stable than the native enzyme at temperatures higher than 65°C. The column reactor was successfully operated for 35 days to produce more than 30% LS using a constant conversion flow system on a pilot plant scale. The present results suggest the possibility of LS production on an industrial scale using a column reactor.