2001 Volume 48 Issue 3 Pages 279-285
Time course of the positional isomers of α-galactobiose in α-linked galactooligosaccharide (α-GOS A) synthesized from galactose by the reverse reaction of α-galactosidase from Candida guilliermondii H-404 was analyzed by using p-aminobenzoic ethyl ester (ABEE)-conversion method. α-Galactobioses in α-GOS A consisted of α-1, 6-, 1, 3-, and 1, 2-galactobioses. α-1, 6-Galactobiose was the main product from an early reaction stage. α-1, 6-, 1, 3-, and 1, 2-Galactobioses were prepared from α-GOS A by an activated carbon chromatography.