Journal of Applied Glycoscience
Online ISSN : 1880-7291
Print ISSN : 1344-7882
ISSN-L : 1344-7882
Production of the Positional Isomers of α-Galactobiose by the Reverse Reaction of α-Galactosidase from Candida guilliermondii H-404
Hiroyuki HashimotoAkiko YamashitaKoji IkuraChie KatayamaMasaru GotoShoichi YasunoMasugu KameiSumio Kitahata
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2001 Volume 48 Issue 3 Pages 279-285

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Abstract

Time course of the positional isomers of α-galactobiose in α-linked galactooligosaccharide (α-GOS A) synthesized from galactose by the reverse reaction of α-galactosidase from Candida guilliermondii H-404 was analyzed by using p-aminobenzoic ethyl ester (ABEE)-conversion method. α-Galactobioses in α-GOS A consisted of α-1, 6-, 1, 3-, and 1, 2-galactobioses. α-1, 6-Galactobiose was the main product from an early reaction stage. α-1, 6-, 1, 3-, and 1, 2-Galactobioses were prepared from α-GOS A by an activated carbon chromatography.

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© The Japanese Society of Applied Glycoscience
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