Cloning and Expression of β-Fructofuranosidase Gene from Arthrobacter sp. K-1

Abstract

A gene encoding an extracellular β-fructofuranosidase (β-FFase), designated bff A, was clonedfrom the chromosomal DNA of Arthrobacter sp. K-1, and expressed in Escherichia coli. The putativebff A consisted of 1734 by encoding β-FFase of 578 amino acids. Comparison of the DNA sequencedata with the N-terminal amino acid sequence of the purified enzyme indicated the presenceof a precursor protein with a signal sequence of 36 residues. The deduced amino acid sequence ofthe β-FFase demonstrated a high degree of homology with that of levansucrase from Acetobacterdiazotrophicus. The N-terminal amino acid sequence and some enzymatic properties of the recombinantenzyme were identical to those of the native enzyme.