Development of simplified evaluation method on heavy metals soil pollution using luminous bacteria 2

— Acute toxicity evaluation of plating metals —

Abstract

Cd, Pb and Cr⁶⁺ have been categorized into Class II Specified Chemical Substances in The Soil Contamination Countermeasures Act of Japan, which have generally been used as plating metals. Fe, Ni, Cu, Zn, Ag and Sn also have widely been used as plating metals. In this study, these 9 plating metals were examined by an acute toxicity test using luminous bacteria (Vibrio fischeri). In the results, it was shown that this bioassay method is available as the screening method for soil pollution based on several legal reference values such as the soil content reference values. In addition, the correlation with the degree of acute toxicity effects on the luminous bacteria with each concentration of plating metals were found and quantified. It could allow the quantitative evaluation of the soil pollution by each of the plating metals.