エアロゾル研究
Online ISSN : 1881-543X
Print ISSN : 0912-2834
ISSN-L : 0912-2834
特集2「次世代のエアロゾル科学・技術を拓く」
大気汚染物質によるスギ花粉アレルゲンCry j 1の化学的修飾と3-ニトロチロシンのHeLa細胞に対するアポトーシス誘導能に関する基礎研究
森田 淳王 青躍龔 秀民仲村 慎一鈴木 美穂中島 拓也関口 和彦中島 大介三輪 誠
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2011 年 27 巻 1 号 p. 71-77

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Recently, prevalence of Japanese cedar pollinosis has been increasing, and it is observed highly in the Kanto area of Japan. As a cause of the high prevalence in Kanto area, we focused attention on 3-nitrotyrosines contained in allergen protein that cause the immune reactions and enhance an immunogenicity of allergen proteins. In this study, we measured the size distribution of airborne Cry j 1 concentrations and the relative quantities of airborne Cry j 1 containing 3-nitrotyrosine; and evaluated an apoptosis inducibility of 3-nitrotyrosine for HeLa cell.
 Cry j 1 were highly observed as fine particles (≦1.1 µm) in the urban atmosphere of Saitama city, Japan. We succeeded for the first time in detecting Cry j 1 containing 3-nitrotyrosine in the urban atmosphere as the probable evidence of chemical modification of Cry j 1 by air pollutants. Cry j 1 that exists in the particle sizes smaller than 7.0 µm contained higher probabilities of 3-nitrotyrosine residues compared with Cry j 1 in the particle sizes larger than 7.0 µm. Moreover, we clarified that 3-nitrotyrosine induces HeLa cell (epidermoid cell) apoptosis.
 From these results, it is suggested that further investigations are needed for the behavior and health effect of Cry j 1 and Cry j 1 containing 3-nitrotyrosine in respirable particles.

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© 2011 日本エアロゾル学会
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