Journal of Oral Tissue Engineering
ORIGINAL ARTICLES
The Effect of Artificial Recombinant Bone Morphogenetic Protein 2 with Titanium-binding Peptide on a Ti Graft in Vivo
Eitoyo KOKUBUKatsutoshi KOKUBUNKenichi MATSUZAKAKenji KASHIWAGIKiyotaka SHIBATakashi INOUEKazuyuki ISHIHARAMasao YOSHINARI
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Volume 12 (2014) Issue 1 Pages 1-7

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Abstract

Surface modification of titanium (Ti) influences cell behavior, and immobilization of bone morphogenetic protein (BMP)-2 on a Ti surface cells to differentiate into osteogenic cells. This study aimed to evaluate the effect of an artificial fusion protein (AFP) comprising a modified BMP-2 and Ti-binding peptide (TBP) motif (AFP-TBP–BMP-2) on osteogenesis of rat abdominal cells on Ti surface in vivo. Ti plates were dipped in three different mixtures of collagen gel: collagen gel only, collagen gel with BMP-2, and collagen gel with AFP-TBP–BMP-2. The treated Ti plates were then implanted into rat abdominal muscles. One and 2 weeks after implantation, these plates were histologically observed. The Ti plate with collagen gel and AFP-TBP–BMP-2 produced cartilage in the muscle at 1 week, and bone-like hard tissue was observed at 2 weeks. These results suggest that the application of collagen gel with AFP-TBP–BMP-2 accelerates osteogenesis in vivo.

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© 2014 by Japanese Association of Regenerative Dentistry
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